Gel Extraction

 Introduction

    In the process of learning new things, sometimes science can become redundant. This week we worked on the Left Flank of our sample and went through the gel extraction process. We started with making a 1.5% gel to run our samples through. To make this we need 30 Ml of 1X TAE and 450 mg of agarose powder. While we waited for our gels to solidify, we tested our samples on the nano-drop. 

From this we got:

 

Ng/Micro-Liters

A260/A280

A260/A230

L1

245.9

2.01

2.04

L2

208.4

2.01

1.39

L3

233.9

2.03

2.07

L4

209.0

2.04

2.09

    After the gels finished solidifying, we added our samples. We ran 18 micro-liters of the DNA sample, and 2 micro-liters of our blue dye. 


    After the gels had finished running their course, we placed them over UV light and carefully removed each sample. 


Featured in the pictures are L3, L4, and our ladder.

 
    Once the samples were extracted, we began the elution process. Adding different buffers, centrifuging them for a minute, then discarding the supernate. 


    After the elution process had finally finished, it was back to the nano-drop to check the results. This second round brought us:

 

Ng/Micro-Liters

A260/A280

A260/A230

L1

8.6

2.49

0.02

L2

5.6

2.39

0.01

L3

15.1

2.22

0.04

L4

12.8

2.27

0.04

    And then for UV-Vis:

 

280

260

230

225

L1

0.11

0.21

7.11

11.00

L2

0.03

0.10

6.32

9.67

L3

0.17

0.35

7.00

10.78

L4

0.14

0.28

4.89

7.71

    Next week we will begin the alcohol precipitation process.

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