Week 1

 Introduction

    Thursday was spent preparing bacteria for whenever the supplies we need arrive. We started by dyeing some samples with Crystal Violet in hopes of it sticking to biomass. After the samples had grown for 48 hours, we evacuated the walls (emptying the trays) and then rinsed them with water, then dumped them out again. After the samples had been successfully evacuated, it was time to apply the dye. We placed 225 micro-liters of Crystal Violet into the samples, allowed them to sit at room temperature for 20 minutes, then repeated the evacuation steps. After the dish has been successfully evacuated, we allow the samples to rest for 24 hours at room temperature being placed at an angle to dry. 


    Come Tuesday, our samples will hopefully reflect the growth of biomass, and we will continue the process of inserting a new fragment into our sample's DNA. 

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